Comment[ArrayExpressAccession]	E-MTAB-4109			
MAGE-TAB Version	1.1			
Investigation Title	Transcriptome analysis of the effect of Perforin on Candida albicans growth			
Comment[AEExperimentDisplayName]	Transcription profiling by array of the effect of Perforin on Candida albicans			
Comment[Submitted Name]	Transcription profiling by array of the effect of Perforin on Candida albicans			
Experiment Description	Perforin is a major effector molecule of human natural killer (NK) cells. It can induce delayed growth of Candida albicans hyphae. Here, the fungal transcriptome was analyzed after a co-incubation with 500ng/µl Perforin.			
Experimental Design	time series design	compound treatment design		
Experimental Design Term Source REF	EFO	EFO		
Experimental Design Term Accession Number	EFO_0001779	EFO_0001755		
Experimental Factor Name	compound	sampling time point	individual	
Experimental Factor Type	compound	sampling time point	individual	
Experimental Factor Term Source REF	EFO	EFO	EFO	
Experimental Factor Term Accession Number	CHEBI_37577	EFO_0000689	EFO_0000542	
Person Last Name	Weber			
Person First Name	Michael			
Person Mid Initials				
Person Email	michael.weber@hki-jena.de			
Person Phone				
Person Fax				
Person Address				
Person Affiliation				
Person Roles	submitter			
Date of Experiment	2015-10-01			
Public Release Date	2020-01-01			
Protocol Name	P-MTAB-47771	P-MTAB-47772	P-MTAB-47773	P-MTAB-47774
Protocol Type	growth protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	nucleic acid extraction protocol
Protocol Term Source REF	EFO	EFO	EFO	EFO
Protocol Term Accession Number	EFO_0003789	EFO_0003813	EFO_0003815	EFO_0002944
Protocol Description	Out from a YPD overnight culture, 1x106 Candida albicans wild type SC5314 cells/ml was grown in RPMI1640 medium with 5% fetal bovine serum (FBS)with or without 500ng/µl Perforin for 2h, 4h and 8h at a temperature of 37°C. RNAs isolated from these conditions were labeld with Cy5. A Cy3-labeled common reference RNA (SC5314 grown for 5h in YPD at 37°C) was used as control.	Sample RNA was labeled with Cy5-CTP and hybridized with a Cy3- labeled common reference RNA on C. albicans DNA microarrays (ClinEuroDiag, Brussels, Belgium)	Slides were hybridized, washed and scanned with a Genepix 4000B (Molecular Devices)	Total fungal RNA was isolated by a hot phenol- chloroform method
Protocol Hardware				
Protocol Software				
Term Source Name	ArrayExpress	EFO		
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/		
Term Source Version				
Comment[AEExperimentType]	transcription profiling by array			
SDRF File	E-MTAB-4109.sdrf.txt