UniqueID (Johnson lab) orf6 # orf19# Name (Candida Annotation Working Group) Annotation (Candida Annotation Working Group) Gene class(es) rfg1/WT #1 rfg1/WT #2 rfg1/WT #3 rfg1/WT #4 F49A8 937 157 hypothetical protein 1.86 3.43 1.79 1.73 J16B11 5934 201 CDC47 DNA helicase and DNA replication licensing factor DNA metabolism/repair | Cell cycle | General metabolism 2.13 1.3 2.61 1.95 J17H12 4293 300 DLD2 mitochondrial D-lactate dehydrogenase Oxidoreductases | Mitochondrial 1.38 2.11 2.51 1.45 F53B10 6279 385 Oxidoreductases | Mitochondrial | General metabolism 1.55 1.55 1.4 1.86 J26E8 2094 413 hypothetical protein 2.32 2.87 1.5 1.72 J1D2 6496 427 RIF1 "RAP1-interacting factor, involved in establishment of repressed chromatin" DNA metabolism/repair | Transcription factors/chromatin/DNA-binding | Stress response | General metabolism 3.08 2.92 2.83 1.59 J18F10 7080 491 SEC63 involved in protein import into ER ER/Golgi/secretion | General metabolism 1.57 1.5 1.78 1.39 M4H2 7005 748 RNA metabolism | General metabolism 1.48 1.43 1.76 1.75 J26H8 1137 797 BAT2 branched-chain amino acid transaminase Amino acid biosynthesis/metabolism/transport | General metabolism 3.09 2.4 1.61 1.7 J16D1 7286 891 AMD1 alpha-mannosidase | AMP deaminase General metabolism 1.9 1.6 1.53 1.56 J33C2 7276 900 General metabolism 1.39 1.42 1.35 1.39 J22G6 566 914 MRS512 involved in fungal growth 2.93 2.08 1.51 1.76 J22B2 8192 943 FET33 Multicopper ferro-O2-oxidoreductase Iron metabolism | Copper metabolism | Oxidoreductases 2.84 1.71 J33A2 8227 978 BDF1 transcription factor Transcription factors/chromatin/DNA-binding 2.27 2.07 1.79 1.34 F58A2 2130 1048 Oxidoreductases | General metabolism 8.18 3.92 J41A11 738 1099 hypothetical protein in Major Repeat Sequence 2.61 2.69 1.78 1.7 F51G1 3472 1120 hypothetical protein 2.23 2.38 1.99 1.73 M12E9 3101 1313 CDR3 opaque-specific multidrug resistance transporter Miscellaneous 1.62 1.96 2.19 Op1G1 4883 1321 HWP1 hyphal wall protein Cell motility/budding/cell division | Cell wall components 5.38 7.04 9.65 J5D10 5290 1358 GCN4 transcriptional activator of the aa starvation response Transcription factors/chromatin/DNA-binding | Amino acid biosynthesis/metabolism/transport | General metabolism 2.77 3.24 1.71 1.23 F57A4 4168 1382 YEA4 golgi uridine diphosphate-N- acetylglucosamine transporter ER/Golgi/secretion | Sugar/polysacchride metabolism | General metabolism 2 2.01 2.95 1.43 F4A7 1336 1613 Amino acid biosynthesis/metabolism/transport | Mitochondrial | General metabolism 1.65 2.17 1.79 1.37 F10B2 1083 1674 CDC53 ubiquitin ligase (cullin) of SCF involved in cell cycle control Cell cycle | General metabolism 1.67 1.92 1.94 1.37 F27C5 4146 1693 Cell motility/budding/cell division 2.51 2.43 1.86 J14E11 3043 1702 ARF3 GTP-binding ADP-ribosylation factor Signaling/kinases/phosphatases 1.69 1.7 1.74 1.49 M4F6 4839 1721 NCE103 conserved hypothetical protein Unknown process and function 7.32 3.77 6.41 1.31 R4H10 4378 1799 Amino acid biosynthesis/metabolism/transport 1.74 1.5 1.5 1.33 J2B7 3432 1825 hypothetical protein 5.55 2.96 6.04 1.23 F25F5 4746 2115 Mitochondrial 1.86 1.79 1.66 1.28 F61A9 4742 2119 NDT80 meiosis-specific protein for exit from pachytene Transcription factors/chromatin/DNA-binding | Cell cycle | General metabolism 2.83 1.74 1.85 1.32 J5F3 1377 2355 ALS10 agglutinin-like protein Cell wall components 3.37 3.27 2.46 8.9 J14H2 1816 ALS3 Cell wall components 3.13 2.71 1.64 15.28 R4B6 4075 2365 POL2 "DNA-directed DNA polymerase epsilon, catalytic subunit A" Transcription factors/chromatin/DNA-binding | DNA metabolism/repair | Stress response | Cell cycle | General metabolism 2.96 2.36 2.26 F33A6 6164 2483 putative telomere binding protein Mitochondrial 1.53 1.45 1.38 1.74 J2F8 2354 2545 DOT6 derepression of telomeric silencing DNA metabolism/repair | Transcription factors/chromatin/DNA-binding | Cell motility/budding/cell division | General metabolism 1.65 1.38 1.48 1.42 J8F8 1438 2633 QUP1 quinate permease Galactose metabolism | Sugar/polysacchride metabolism 1.41 1.64 1.73 F53A7 1258 2649 PCL1 G1 cyclin that associates with PHO85 Cell cycle | Signaling/kinases/phosphatases 3.29 2 4.78 1.27 F14B2 6585 2767 hypothetical protein Cell wall components 1.36 1.78 1.78 1.61 F11C6 4943 2849 AQY1 aquaporin Miscellaneous 6.5 4.47 2.16 2.63 J30F6 4383 2873 Transcription factors/chromatin/DNA-binding | DNA metabolism/repair | Cell cycle | General metabolism 2.21 2.13 1.59 1.98 J6D12 6668 2948 SNO1 glutamine amidotransferase General metabolism 2.25 2.16 1.74 1.22 F9A8 2690 3159 hypothetical protein RNA metabolism | General metabolism 3.19 3.11 F14H9 4392 3207 CCN1 G1 cyclin Cell cycle | Signaling/kinases/phosphatases 2.63 3.45 4.6 1.86 J5G4 2886 3374 ECE1 secreted cell elongation protein 8.24 6.95 4.22 21.07 J27F10 2793 3447 PBN1 required for post-translational processing of the protease B precursor Prb1p ER/Golgi/secretion | General metabolism 1.73 2.04 1.29 1.74 F5F9 4977 3482 NPY1 hydrolyzes the pyrophosphate linkage in NADH and related nucleotides | NADH pyrophosphatase General metabolism 1.68 1.57 1.88 1.37 J18B9 1877 3490 MRS512 Major Repeat Sequence 2.65 2.19 1.76 1.85 F15A8 4367 3507 MCR1 cytochrome b5 reductase Oxidative stress | Lipid metabolism | Stress response | Oxidoreductases | Mitochondrial | General metabolism 1.44 1.94 1.62 F56B9 3282 3612 PST2 "NADH:quinone oxidoreductase | 1,4-benzoquinone reductase" Unknown process and function 3.84 4.84 2.2 F33A1 2877 3700 TOM70 mitochondrial outer membrane specialized import receptor Mitochondrial | General metabolism 2.06 1.87 2.23 1.89 J43H2 3954 3765 RAX2 membrane protein involved in bipolar budding Cell motility/budding/cell division | Cell wall components 1.51 1.82 1.88 2.54 J5D9 5246 3856 CDC28 cell division control protein Cell cycle | Signaling/kinases/phosphatases | General metabolism 2.39 1.76 1.43 1.49 F52B5 550 3869 hypothetical protein 1.62 2.8 3.48 1.46 J46D8 7452 4040 Amino acid biosynthesis/metabolism/transport | Mitochondrial | General metabolism 1.66 1.71 1.78 1.51 F53A1 1668 4216 Oxidative stress | Stress response | Cell wall components | Osmotic stress | General metabolism 19.14 25.08 2.92 1.51 J42D4 6612 4233 THR4 threonine synthase Amino acid biosynthesis/metabolism/transport | General metabolism 1.38 1.47 1.54 1.7 J16C5 3107 4279 MNN1 mannosyltransferase ER/Golgi/secretion | Sugar/polysacchride metabolism | General metabolism 8.04 7.6 1.9 J5C9 4614 4309 GRP3 induced by osmotic stress Stress response | Oxidoreductases 3.52 3.92 1.83 R4B8 3258 4328 CCC2 copper-transporting ATPase ER/Golgi/secretion | Copper metabolism 1.68 1.65 2.14 1.73 F14B6 3992 4348 RGR1 transcriptional regulation Transcription factors/chromatin/DNA-binding | General metabolism 1.83 1.78 2.15 1.31 J6F2 4542 4377 KRE1 "cell wall beta-1,6-glucan assembly" 2.66 2.5 3.31 1.85 J20F10 4311 4476 IFD6 aryl-alcohol dehydrogenase Oxidoreductases | General metabolism 1.61 1.82 1.34 1.49 J19H10 4310 4477 IFD4 aryl-alcohol dehydrogenase Oxidoreductases | General metabolism 11.81 8.79 3.16 1.81 J18B10 2884 4485 MRS512 Major Repeat Sequence 2.51 3.01 1.79 1.82 J24F2 7915 4616 POL30 accessory factor for DNA polymerase delta | Proliferating Cell Nuclear Antigen (PCNA) DNA metabolism/repair | Stress response | Cell cycle | General metabolism 2.03 1.66 1.53 1.59 R4G1 3347 4650 ILV6 regulatory subunit of acetolacetate synthase Amino acid biosynthesis/metabolism/transport | Mitochondrial | General metabolism 1.73 1.55 1.59 1.38 J45F2 1767 4688 4.42 4.68 4.86 3.49 F24F12 3152 4716 GDH3 NADP-glutamate dehydrogenase Amino acid biosynthesis/metabolism/transport | Oxidoreductases | General metabolism 1.65 1.95 3.01 1.67 F7A2 4019 4749 hypothetical membrane protein 2.13 1.98 4.33 2.72 J7F4 5211 4805 RSN1 membrane transporter Unknown process and function 1.56 1.46 1.82 1.6 J17F1 7358 4853 HCM1 fork head family transcription factor Cell motility/budding/cell division | Transcription factors/chromatin/DNA-binding | Cell cycle | General metabolism 4.16 2.31 2.58 1.16 F9B2 7346 4865 Lipid metabolism | ER/Golgi/secretion | General metabolism 1.79 2.02 1.21 1.83 F4A12 1608 5061 ADE5 phosphoribosylamine-glycine ligase and phosphoribosylformylglycinamidine cyclo-ligase General metabolism 1.54 1.76 1.84 2.03 J25B2 6300 5076 PFY1 profilin Cell motility/budding/cell division | Stress response | Osmotic stress 2.13 1.7 1.45 2.01 R4A7 6053 5188 Stress response | Osmotic stress 1.6 2.32 2.78 1.34 J18B8 3502 5191 hypothetical protein 2.81 2.5 1.44 2.3 M4G7 4127 5557 MNN43 transfer mannosylphosphate to oligosaccharides Stress response | Sugar/polysacchride metabolism | General metabolism 5.35 1.32 4.88 1.95 Op1B8 5585 Secreted/degradative enzymes | General metabolism 4.4 10.6 J22D2 4438 5596 CAF4 WD-repeat protein 2.11 1.56 1.91 J40H4 4779 5620 Unknown process and function 3.22 2.37 1.93 1.73 F13H2 4780 5621 conserved hypothetical protein Unknown process and function 2.41 2.25 1.89 1.41 J4F7 3803 5716 SAP4 candidapepsin 4 precursor Secreted/degradative enzymes | General metabolism 4.71 4.01 10.81 J31H4 6198 5760 IHD1 Induced in Hyphal Development | membrane protein Secreted/degradative enzymes | Cell wall components | Sugar/polysacchride metabolism 1.45 2.48 2.72 3.27 J21F8 6183 5775 hypothetical protein 2.72 2.43 1.7 1.74 F33E10 4851 5779 RNR1 ribonucleotide reductase large subunit DNA metabolism/repair | Cell cycle | Oxidoreductases | General metabolism 4.72 4.69 3.17 1.67 J42B6 6639 5805 DLD3 mitochondrial D-lactate ferricytochrome c oxidoreductase Oxidoreductases | Mitochondrial | General metabolism 3.45 3.92 6.18 1.93 J32B12 6644 5811 Amino acid biosynthesis/metabolism/transport | General metabolism 6.24 6.53 1.82 J19G4 6139 5839 PDR17 lipid biosynthesis and multidrug resistance Lipid metabolism | General metabolism 1.68 1.72 2 1.27 J2B2 7604 6060 GCN20 "ATP-binding cassette (ABC) family, regulator of translational elongation" General metabolism 1.38 1.55 2.21 1.65 R4E7 6241 6100 CRD2 cardiolipin synthase Lipid metabolism | Mitochondrial | General metabolism 1.71 1.85 1.54 1.31 J34C10 6237 6105 Lipid metabolism | General metabolism 3.2 4.73 2.86 2.94 Op1A5 6232 6109 TUP1 general transcription repressor Transcription factors/chromatin/DNA-binding | General metabolism 3.58 1.76 1.99 J6B7 537 6202 PRY4 "pathogenesis related protein, repressed by TUP1 protein 4" Secreted/degradative enzymes | ER/Golgi/secretion | Vacuolar 5.23 3.54 3.56 1.41 F24A2 5521 6328 ACN9 conserved hypothetical protein Sugar/polysacchride metabolism | Mitochondrial | General metabolism 1.66 1.71 2.25 1.69 J22D1 7557 6569 NUP2 purine nucleoside permease 2.94 2 2.74 1.48 J10D1 7558 6570 NUP3 purine nucleoside permease 9.68 13.02 4.69 2.08 J46C2 6764 6656 1.5 1.67 2.68 1.6 R4H4 6805 6696 TIM9 mitochondrial inner membrane translocase Mitochondrial | General metabolism 2.61 2.66 4.07 1.57 J29F11 8881 6749 Amino acid biosynthesis/metabolism/transport | RNA metabolism | General metabolism 1.51 1.37 1.56 1.63 F17D6 8848 6783 MRS6 geranylgeranyltransferase regulatory subunit Signaling/kinases/phosphatases 1.98 2.44 2.42 1.17 F17D10 8845 6786 conserved hypothetical protein 2.85 2.61 1.46 J34A1 3405 6826 2.03 1.86 2.62 1.5 J34A3 7140 6877 5.21 6.25 1.46 1.69 M11G7 7316 6930 conserved hypothetical protein 2.41 1.34 1.97 1.5 J23H6 7722 6976 multidrug efflux protein Unknown process and function 4.56 4.25 3.72 1.2 J26H1 7931 7005 MRS512 Major Repeat Sequence 3.36 2.45 1.73 2.07 R4D8 7942 7016 vacuolar endopolyphosphatase Vacuolar | General metabolism 1.66 2.3 1.72 1.32 F4B8 8500 7053 GAC1 regulatory subunit for phosphoprotein phosphatase type 1 (PP-1) Transcription factors/chromatin/DNA-binding | Stress response | Cell cycle | Signaling/kinases/phosphatases | Sugar/polysacchride metabolism | General metabolism 2.79 1.93 2.18 J39D5 8433 7119 DNA metabolism/repair | Transcription factors/chromatin/DNA-binding | Stress response | Cell cycle | General metabolism 1.46 1.57 1.53 1.33 F34F5 8163 7263 similarity to X-Pro dipeptidases Miscellaneous 3.66 2.91 2.49 1.41 F28D7 8509 7349 CHS4 chitin synthase regulatory factor Stress response | Sugar/polysacchride metabolism | General metabolism | Osmotic stress 2.66 2.54 1.92 1.58 J14D6 8514 7354 LAC2 longevity-assurance protein Lipid metabolism | ER/Golgi/secretion | General metabolism 2.67 2.05 2.49 1.2 J16E12 8523 7363 ER/Golgi/secretion | Sugar/polysacchride metabolism | General metabolism 1.35 1.71 1.46 1.62 F9A11 8528 7368 PUB1 polyadenylated RNA-binding protein | not reported to associate with polyribosomes RNA metabolism | General metabolism 1.73 1.83 1.69 1.38 J43D2 8543 7383 MNN9 required for complex glycosylation ER/Golgi/secretion | Sugar/polysacchride metabolism | General metabolism 1.48 1.6 1.88 1.75 J35B10 8569 7409 ERV25 component of COPII coat of ER- derived vesicles | p24 protein family ER/Golgi/secretion 1.66 1.47 1.49 1.8 J19E6 8585 7425 UNG1 uracil DNA glycosylase DNA metabolism/repair | Stress response | Sugar/polysacchride metabolism | Mitochondrial | General metabolism 2.27 1.97 2.09 1.55 J22F4 8586 7426 OST6 subunit of N- oligosaccharyltransferase complex ER/Golgi/secretion | Sugar/polysacchride metabolism | General metabolism 2.11 1.72 1.77 1.97 F48E10 8756 7504 conserved hypothetical protein 5.03 3.2 J32A4 8931 7583 potential fungal Zn(2)-Cys(6) binuclear cluster domain 2.08 1.55 1.54 1.61 M4E3 8940 7592 FAA4 long-chain fatty acid--CoA ligase and synthetase 4 Lipid metabolism | General metabolism 2.02 1.61 2.31 1.33 J2A4 8958 7610 PTP3 protein tyrosine phosphatase Transcription factors/chromatin/DNA-binding | Signaling/kinases/phosphatases | Osmotic stress | General metabolism 3.25 2.61 1.54 1.66 F49A5 8973 7625 KRE13 KRE1 protein precursor 1.76 1.86 1.86 1.29 J36F6 no orf6 1.55 1.6 1.35 2.16 SAM False Discovery Rate (FDR) = 3.24%