UniqueID (Johnson lab) orf6# orf19# Name (Candida Annotation Working Group) Annotation (Candida Annotation Working Group) Gene class(es) tup1/WT #1 tup1/WT #2 tup1/WT #3 tup1/WT #4 tup1/WT #5 tup1/WT #6 M12E2 1559 86 GPX2 glutathione peroxidase Oxidative stress | Stress response | Oxidoreductases | General metabolism 3.14 2.964912281 3.42 1.91 2.39 2.55 J51D3 3706 94 hypothetical protein 3.68 3.82 3.71 J50G11 3481 133 hypothetical protein 3.88 2.26 3.21 3.09 J43A12 5940 207 Cell motility/budding/cell division | Cell wall components 2.63 4.16 1.58 3.65 4.88 10.13 F19E7 1271 244 DCG1 involved in nitrogen- catabolite metabolism General metabolism 4.41 2.71 1.47 3 2.36 5.52 J13C11 1272 245 DDC1 DNA damage checkpoint protein Cell cycle 4.49 1.98 2.61 1.95 F19A6 4807 278 neutral amino acid transport system protein 4.41 3.29 6.64 2.8 10.59 2.05 M12C9 4292 301 "hypothetical protein, likely cell wall localized and GPI-anchored" Cell motility/budding/cell division | Cell wall components 4.04 10.94 3.01 J29F4 6743 532 Secreted/degradative enzymes | Cell wall components 2.31 5.95 3.9 J50G7 449 535 Cell wall components 132.93 34.1 167.38 16.01 133.07 20.54 F51C9 425 629 aryl-alcohol dehydrogenase Oxidoreductases | General metabolism 19.23 3.73 9.9 3.95 J2F4 1985 690 PLB1 lysophospholipase Lipid metabolism | Secreted/degradative enzymes | General metabolism 4.92 3.41 3.75 4.06 J16F1 3325 701 FRE8 ferric reductase Iron metabolism | Oxidoreductases 6.45 32.7 43.4 J14E6 2637 716 similar to pore-forming bacterial Septicolysin 9.43 3.45 7.27 9.14 R4G12 6020 723 USV1 conserved hypothetical protein Unknown process and function 1.78 4.43 2.85 4.83 3.71 15.7 F50E2 4136 747 NBP35 nuclear ATPase Miscellaneous 2.89 2.59 2.12 3.88 J5C3 3635 756 SAP7 secreted aspartyl proteinase 7 Secreted/degradative enzymes | General metabolism 62.2 59.41 65.4 J43C8 900 849 MNN4 regulator of cell wall mannosyl phosphorylation Stress response | Sugar/polysacchride metabolism | General metabolism 14.15 10.13 66.56 16.38 F15B11 1543 851 MNN41 regulator of cell wall mannosyl phosphorylation Stress response | Sugar/polysacchride metabolism | General metabolism 5.9 6.21 11.22 10.22 19.93 J9H4 1355 854 General metabolism 2.94 8.12 4.13 3.53 5.09 5.86 F46H8 7281 896 CHK1 histidine kinase osmosensor | two-component singal transducer Oxidative stress | Transcription factors/chromatin/DNA-binding | Stress response | Signaling/kinases/phosphatases | Osmotic stress | General metabolism 14.41 2.64 5.3 3.54 9.49 J17C11 2321 1036 MNS1 "mannosyl- oligosaccharide 1,2-alpha-mannosidase" ER/Golgi/secretion | Sugar/polysacchride metabolism | General metabolism 2.03 1.6 1.78 1.74 1.56 1.61 F58B11 2319 1038 hypothetical protein 8.14 3.62 5.93 3.82 6.9 6.13 F58A2 2130 1048 Oxidoreductases | General metabolism 13.83 3.75 2.83 9.4 F51G1 3472 1120 hypothetical protein 11.62 57.88 19.79 16.23 33.09 36.75 J26G3 780 1130 hypothetical protein 12.14 5.57 4.08 6.89 2.65 J16D9 5730 1263 FRE1 ferric reductase Iron metabolism | Copper metabolism | Oxidoreductases 2.32 4.01 10.03 J17C7 5731 1264 FRE2 ferric reductase Iron metabolism | Oxidoreductases 8.09 17.55 10 33.32 10.1 14 F53C5 2162 1270 FRE3 ferric reductase Iron metabolism | Copper metabolism | Oxidoreductases 2.56 3.65 5.72 5.32 8.98 F23C12 1385 1302 3.65 4.97 7.85 F55E9 4883 1321 HWP1 hyphal wall protein Cell motility/budding/cell division | Cell wall components 89.39 63.5 116.78 75.5 71.21 34.63 J2C2 4889 1327 RBT1 "repressed by Tup1, related to HWP1" Cell motility/budding/cell division | Cell wall components 26.29 30.8 63.53 70.93 31.07 35.9 J2C10 6384 1415 FRE10 ferric reductase Iron metabolism | Oxidoreductases 5.39 18.81 7.63 36.25 7.07 18.88 F25C10 6419 1562 hypothetical protein 3.08 3.13 1.78 F65A6 5334 1586 PLC3 phosphatidylinositol phospholipase C 4.42 2.58 7.73 4.86 5.03 5.23 F46C2 1648 1599 hypothetical protein 4.49 4.56 4.13 J26F5 1649 1600 hypothetical protein 3.22 4.5 2.65 1.78 2.66 2.62 J19G1 2332 1648 RAD50 DNA repair protein Transcription factors/chromatin/DNA-binding | DNA metabolism/repair | Stress response | Cell cycle | General metabolism 2.89 1.81 1.61 1.46 F27G5 3042 1701 RKI1 ribose-5-phosphate ketol-isomerase Sugar/polysacchride metabolism | General metabolism 2.82 6.83 1.94 8.91 2.7 13.74 F27G7 3043 1702 ARF3 GTP-binding ADP-ribosylation factor Signaling/kinases/phosphatases 5.21 4.07 1.92 2.47 2.12 2.49 F1A3 1326 1822 UME6 transcriptional regulator of early meiotic genes Transcription factors/chromatin/DNA-binding | Cell cycle | General metabolism 16.61 7.98 5.88 7.57 4.6 10.04 F57E9 3432 1825 hypothetical protein 2.97 2.45 1.65 F36G1 7054 1866 VMA10 vacuolar ATPase V1 domain subunit G Vacuolar | Sugar/polysacchride metabolism | General metabolism 5.05 2.28 2.17 2.26 1.84 2.43 F58G3 1281 1930 FRE4 ferric reductase Iron metabolism | Oxidoreductases 17.32 24.48 26.56 43.77 22.71 J16G7 5606 1932 Iron metabolism | Copper metabolism | Oxidoreductases 15.37 14.05 18.49 7.81 17.46 J48C8 4255 2210 2.9 2.52 2.64 2.43 F66E10 3567 2274 hypothetical protein 3.14 5.16 1.85 F36G3 4206 2346 highly conserved hypothetical protein Mitochondrial 2.3 1.91 1.71 1.93 J5F3 1377 2355 ALS10 agglutinin-like protein Cell wall components 32.97 9 59.77 10.18 39.24 14.31 J14H2 1816 ALS3 Cell wall components 37.76 8 80.3 5.75 35.39 10.08 F63H1 8497 2445 Amino acid biosynthesis/metabolism/transport 2.67 13.59 8.12 J26B4 3561 2459 hypothetical protein Miscellaneous 2.65 2.27 3.49 3.76 J4H5 4968 2467 PRN1 RNA pol II transcription cofactor 2.22 3.07 3.44 F19A9 3817 2646 HAP4 positive regulator of cytochrome C genes CYC1 and CYC7 Cell motility/budding/cell division | ER/Golgi/secretion | Stress response | Osmotic stress 2.78 2.88 2.8 J6F5 5481 2693 URE1 nitrogen catabolite repression regulator and prion generator Transcription factors/chromatin/DNA-binding | General metabolism 6.06 4.09 6.66 F22G6 4494 2833 hypothetical protein 10.49 14.17 17.85 F31G10 6119 2903 conserved hypothetical protein 9.08 2.53 4.04 1.8 4.6 3.31 F58F8 6664 2952 EXG1 "exo-1,3-beta-glucanase" Cell wall components | Sugar/polysacchride metabolism | General metabolism 5 2.53 7.24 F68F6 7755 3018 conserved hypothetical protein DNA metabolism/repair | Transcription factors/chromatin/DNA-binding | General metabolism 10.06 4.56 14.23 2.38 20.21 6 F47E3 8193 3065 2.83 5.52 1.8 J43C4 6935 3110 Stress response | Sugar/polysacchride metabolism | General metabolism 7.74 3.67 2.66 2.84 5.59 4.49 J32A10 6926 3117 32.18 2.96 8.95 2.29 14.68 10.15 F19B4 5864 3127 CZF1 zinc finger protein Transcription factors/chromatin/DNA-binding | Cell cycle | General metabolism 3.22 3.12 3.36 3.94 F14H9 4392 3207 CCN1 G1 cyclin Cell cycle | Signaling/kinases/phosphatases 1.83 1.7 1.92 1.72 2.63 3.17 J43C6 1765 3221 CPA2 "carbamoyl phosphate synthetase large subunit, arginine biosynthesis" Amino acid biosynthesis/metabolism/transport | General metabolism 3.11 3.67 3.06 10.7 3.3 5.29 F16E8 8282 3282 hypohetical protein 5.67 5.89 6.09 F23E5 4731 3340 SOD2 manganese-superoxide dismutase Oxidoreductases | Mitochondrial | General metabolism 1.83 1.67 1.94 3 1.66 2.6 J5G4 2886 3374 ECE1 secreted cell elongation protein 53.49 78.47 7 91.15 43.15 F3C1 2936 3376 6.41 16.62 7.84 J2G4 2933 3380 conserved hypothetical protein Cell motility/budding/cell division | Cell wall components 46.24 54.06 109.08 414.67 89.78 112.08 F18B5 6466 3439 hypothetical protein 2.43 3.84 2.17 4.16 2.91 4.15 F57F5 994 3460 hypothetical protein 3.85 6.37 18.88 5.24 18.57 18.25 J50F2 3764 3461 3.44 2.71 8.98 3.47 4.31 3.73 F66C1 2641 3643 hypothetical protein 3.63 5.23 2.82 J9B10 6479 3668 HGT2 hexose transporter Signaling/kinases/phosphatases 1.92 10 13.89 13.5 8.03 21 J21F6 6481 3670 GAL1 galactokinase Transcription factors/chromatin/DNA-binding | Galactose metabolism | Sugar/polysacchride metabolism | General metabolism 4.66 5.67 8.65 5.93 4.27 19.62 J17A4 6483 3672 GAL10 UDP glucose-4-epimerase Galactose metabolism | Sugar/polysacchride metabolism | General metabolism 1.44 F41C4 6485 3674 GAL102 UDP-glucose 4-epimerase Galactose metabolism | Sugar/polysacchride metabolism | General metabolism 4.29 7.34 16.41 7.66 33.13 J19E5 6486 3675 GAL7 galactose-1-phosphate uridyl transferase Galactose metabolism | Sugar/polysacchride metabolism | General metabolism 3.42 8.24 10.57 12.25 3.8 42.59 F67C10 1691 3733 IDP2 NADP-dependent isocitrate dehydrogenase Amino acid biosynthesis/metabolism/transport | Oxidoreductases | General metabolism 4.02 2.42 2.89 5 2.21 6.25 F64E12 7518 3823 ZDS1 involved in negative regulation of cell polarity Cell motility/budding/cell division | Transcription factors/chromatin/DNA-binding | DNA metabolism/repair | General metabolism 1.57 1.72 1.92 2.58 1.71 2.08 Op1H3 3829 Miscellaneous 7.5 5.86 7.74 9.17 F41C10 7538 3843 SEC66 signal recognition particle receptor | ER protein-translocation complex subunit ER/Golgi/secretion | General metabolism 5.79 4.75 1.89 3.84 2.87 8.62 F55G1 2313 3852 "putative alpha-1,2-mannosidase" 17.91 10.4 7.16 F9C12 5146 3902 conserved hypothetical protein 27.26 20.46 41.77 35.21 42.17 J40B10 4050 3926 RNY1 ribonuclease from the T2 family Cell motility/budding/cell division | Secreted/degradative enzymes | RNA metabolism 12.47 9.74 22.52 F25A4 1915 3931 SFC1 mitochondrial succinate-fumarate transporter Mitochondrial 1.11 4.33 4.14 6.86 2.65 3.5 F60G3 1914 3932 conserved hypothetical protein 16.36 2.76 4.87 1.77 5.75 4.67 F30G9 2297 3934 CAR1 arginase Amino acid biosynthesis/metabolism/transport | General metabolism 2.91 8.29 2.13 3.57 2.67 8.12 J4E9 5658 3982 alpha-glucosidase maltase Sugar/polysacchride metabolism 27.32 7.38 9.87 J6H3 6854 4082 DDR48 flocculent specific protein | contains NNDDNSYG motif DNA metabolism/repair | Stress response | General metabolism 48.66 18 60.95 5.46 72.7 27.2 F26F10 5229 4150 glutaredoxin Vacuolar 2.98 3.3 1.56 3.31 2.42 6.42 J42G8 2620 4156 hypothetical protein 2.65 1.8 2.61 2.51 1.78 2.8 J3F8 790 4212 FET31 multicopper ferro-O2- oxidoreductase involved in high-affinity iron uptake Iron metabolism | Copper metabolism | Oxidoreductases 1.79 4.2 3 J19B6 6610 4235 PDE1 cyclic nucleotide phosphodiesterase Signaling/kinases/phosphatases 1.95 2.06 1.9 2.47 J26G2 6599 4246 Mitochondrial 5.83 3.12 4.58 3.99 5.3 3.91 F30G1 3969 4255 PST1 protoplast-secreted Miscellaneous 44.25 8.83 10.43 31.21 F15C9 3492 4265 UAP1 UDP-n- acetylglucosamine pyrophosphorylase General metabolism 3.9 5.52 1.39 3.31 3.54 4.21 J3B8 3112 4274 PUT1 proline oxidase Amino acid biosynthesis/metabolism/transport | Oxidoreductases | Mitochondrial | General metabolism 2.27 8.34 2.3 4.04 J5C9 4614 4309 GRP3 induced by osmotic stress Stress response | Oxidoreductases 25.92 11.48 14.27 F20C2 3168 4316 conserved hypothetical protein Mitochondrial 5.56 3.28 2.05 3.58 3.54 5.04 F54E6 1698 4334 hypothetical protein Cell motility/budding/cell division | Cell wall components 19.51 26.4 7.88 9.51 J24D5 4197 4504 Oxidoreductases | General metabolism 11.18 9.67 16.71 2.25 9.65 13.45 J19C3 5005 4506 Amino acid biosynthesis/metabolism/transport | General metabolism 2.06 4.56 2.34 3.74 3.58 4.38 J13G11 6673 4527 Galactose metabolism | Sugar/polysacchride metabolism 1.55 10.03 8.97 7.8 5.45 29 J36B9 4600.1 2.85 1.9 1.49 2.18 1.99 2.15 J3G3 7905 4606 Lipid metabolism | General metabolism 4.85 5.57 7.37 F30H8 7906 4607 5.24 1.94 6.25 5.27 F51C1 2053 4630 CPA1 "carbamoyl phosphate synthetase, arginine specific" Amino acid biosynthesis/metabolism/transport | General metabolism 1.62 2.01 2.53 1.68 F12G6 4600 4755 KEX2 Kexin protease | late Golgi endoprotease that processes of alpha-factor ER/Golgi/secretion | General metabolism 2.95 3.1 1.98 2.26 3.58 3.74 M12E8 4590 4765 CCW12 cell wall mannoprotein Cell wall components 3.62 5.34 3.19 6.27 5.28 12.33 M4A7 4898 4788 "ARG5,6" N-acetyl-gamma-glutamyl-phosphate reductase and acetylglutamate kinase | N-acetyl-gamma-glutamyl-phosphate reductase and acetylglutamate kinase | acetylglutamate kinase and acetylglutamyl-phosphate reductase Amino acid biosynthesis/metabolism/transport | Oxidoreductases | Mitochondrial | General metabolism 6.39 14.05 3.34 11.29 9.13 12.93 F63B11 5799 4899 ER/Golgi/secretion | Sugar/polysacchride metabolism | General metabolism 54.27 27.75 28.62 J43B11 2025 4900 MNN13 mannosyltransferase Sugar/polysacchride metabolism | General metabolism 2.4 3.4 4.19 5.9 6.11 20.57 F61D9 3143 4975 Cell motility/budding/cell division | Cell wall components 61.46 56.38 17 82.21 29.8 F67A5 8009 5000 CYB3 cytochrome b2 precursor Oxidoreductases | Mitochondrial | General metabolism 2.32 2.07 2.38 2.14 F49B9 1609 5063 4.85 4.55 2.2 1.25 3.25 3.47 F10B5 2427 5069 1.96 7.33 4.19 6 2.56 9.3 J48B7 6282 5094 BUL3 ubiquitin-mediated protein degradation General metabolism 8.99 6.82 3.12 6.98 F55F10 6340 5110 conserved hypothetical protein Cell cycle | Vacuolar 1.54 1.75 2.98 1.66 1.83 J24C9 5956 5142 DFR1 dihydrofolate reductase Oxidoreductases | General metabolism 2.6 2.13 1.83 1.38 J2G10 3453 5210 XBP1 transcription factor Transcription factors/chromatin/DNA-binding | Stress response 14.42 6.3 6.78 F44A12 7677 5265 KIP4 kinesin heavy chain homolog Cell motility/budding/cell division | Cell cycle 1.79 2.88 3.23 5.5 2.2 M4C11 8683 5447 sugar transporter Sugar/polysacchride metabolism 7.19 5 10.82 4.04 8.35 7.56 J43A11 4127 5557 MNN43 transfer mannosylphosphate to oligosaccharides Stress response | Sugar/polysacchride metabolism | General metabolism 18.01 11.2 24.44 F49G2 3244 5567 POP4 rRNA and tRNA processing RNA metabolism | General metabolism 6.8 4.81 6.44 3.51 F11C10 5062 5610 ARG3 ornithine carbamoyltransferase Amino acid biosynthesis/metabolism/transport | General metabolism 14.06 5.51 4.47 8.08 J19G9 4503 5634 FRP1 ferric reductase Iron metabolism | Oxidoreductases 9.23 8.24 5.29 12.27 5.02 F1E12 4504 5635 RBT6 glycosyl- phosphatidylinositol protein | similar to RBT5 14.35 16.67 6.48 18.67 8.46 12 F49E3 4505 5636 RBT5 repressed by TUP1 protein 5 Cell wall components 63.45 48.43 40.05 17.97 43.29 J31H4 6198 5760 IHD1 Induced in Hyphal Development | membrane protein Secreted/degradative enzymes | Cell wall components | Sugar/polysacchride metabolism 1.74 2.1 3.9 1.93 2.46 F38B4 6186 5772 actin overexpression resistant Unknown process and function 1.85 1.7 1.34 1.59 M12A10 6631 5797 PLC2 phosphatidylinositol phospholipase C 2.09 1.41 4.16 2.39 2.84 3.01 F54E10 9116 5921 similarity to peroxisomal membrane carrier protein Lipid metabolism | General metabolism 1.79 2.39 1.49 2.26 F61E9 9113 5924 conserved hypothetical protein Cell motility/budding/cell division | Stress response | Osmotic stress 4.23 5.3 8.88 4.19 J1A6 3156 6028 CLN2 G1 cyclin Cell cycle | Signaling/kinases/phosphatases 7.94 10.93 13.43 J39A12 1065 6079 hypothetical protein 10.73 2.07 1.88 2.95 5.77 2.92 F35G1 6258 6083 hypothetical protein 3.12 1.81 5.75 2.03 F63F5 5666 6148 USO2 intracellular protein transport ER/Golgi/secretion | General metabolism 17.88 5.45 8.66 4.61 5.23 J6B7 537 6202 PRY4 "pathogenesis related protein, repressed by TUP1 protein 4" Secreted/degradative enzymes | ER/Golgi/secretion | Vacuolar 8.72 6.18 10.5 10.22 10.28 17.31 J27G10 7834 6420 hypothetical membrane protein with repeated hydrophobic/hydrophilic domains Cell wall components 2.49 14.01 7.88 4.04 5.64 J17A8 7648 6512 EXO70 70 kDa exocyst component protein Cell motility/budding/cell division | ER/Golgi/secretion 1.78 2.12 1.69 1.68 2.2 1.54 J7B4 7624 6536 IQG1 RAS GTPase-activating-like Cell motility/budding/cell division | Stress response | Osmotic stress 1.58 1.95 2.99 J10C3 7556 6568 RHC18 involved in recombination repair DNA metabolism/repair | Stress response | General metabolism 3.3 3.8 1.29 3 1.66 3.41 F11G3 6814 6705 conserved hypothetical protein Unknown process and function 2.19 3.97 2.83 6.73 2.92 14.43 F40C9 6987 6838 SPS24 "peroxisomal 2,4- dienoyl-CoA reductase" Lipid metabolism | Oxidoreductases | General metabolism 2.02 2.05 2 2.59 F40C8 6993 6844 ICL1 isocitrate lyase General metabolism 10.16 12 7.98 20 7.53 8 F64B8 7714 6968 conserved hypothetical protein 2.13 1.96 2.37 1.65 4.3 3.07 J3A12 8459 7094 SNF32 glucose sensor or transporter protein Signaling/kinases/phosphatases 7.24 21.95 11.3 7.67 15.58 36.34 F68F10 8090 7190 OGG1 8-oxoguanine DNA glycosylase | DNA repair DNA metabolism/repair | Stress response | Sugar/polysacchride metabolism | Mitochondrial | General metabolism 4.35 4.16 2.22 4.33 F66C9 8119 7219 FTR1 plasma membrane iron permease Iron metabolism 2.82 4.49 2.49 4.95 2.04 2.12 J19G10 8377 7296 conserved hypothetical protein 2.1 2.2 5.99 5.46 3.58 8.99 J43E4 8384 7303 conserved hypothetical protein 1.82 1.44 1.87 1.38 F38E8 8404 7323 CBP1 corticosteroid- binding protein Amino acid biosynthesis/metabolism/transport | Oxidoreductases | General metabolism 4.38 3.7 1.58 6.01 J30G7 8511 7350 Unknown process and function 5.18 4.02 2.94 2.64 3.71 6.22 J19E6 8585 7425 UNG1 uracil DNA glycosylase DNA metabolism/repair | Stress response | Sugar/polysacchride metabolism | Mitochondrial | General metabolism 2.27 2.06 2.19 2.02 1.81 3.2 J22D10 8594 7434 GLG2 glycogenin | self-glucosylating initiator of glycogen synthesis Sugar/polysacchride metabolism | General metabolism 2.03 2.54 2.67 1.98 2.39 3.54 J18H1 8608 7448 LYS9 "seventh step in lysine biosynthesis pathway | saccharopine dehydrogenase (NADP+, L-glutamate forming)" Amino acid biosynthesis/metabolism/transport | Oxidoreductases | General metabolism 1.89 2.18 2.02 1.43 2.05 2.07 F38B8 8721 7469 ARG1 argininosuccinate synthetase Amino acid biosynthesis/metabolism/transport | General metabolism 10.37 12.37 10 16.36 22.34 F48E11 8754 7502 hypothetical protein 5.1 2.74 5.32 3.74 6.21 3.31 F48E10 8756 7504 conserved hypothetical protein 4.87 3 2.17 2.3 2.83 2.9 F48E3 8766 7514 PCK1 phosphoenolpyruvate carboxykinase Sugar/polysacchride metabolism | General metabolism 5.43 2.22 34.84 39 3.6 20.1 F47H8 8911 7563 BET2 geranylgeranyltransferase type II beta subunit General metabolism 2.14 1.63 1.33 2.28 M12A12 9016 7668 MAL2 alpha-glucosidase (maltase) Sugar/polysacchride metabolism 6.15 3.25 3.77 3.45 3.36 6.58 F65A12 9.26 2.1 2.77 2.26 7.76 2.7 SAM False Discovery Rate (FDR) = 0.15%