Comment[ArrayExpressAccession]	E-MTAB-2653
MAGE-TAB Version	1.1																				
Investigation Title	Characterization of yap7 in Saccharomyces cerevisiae and the human pathogen Candida glabrata																				
Comment[Submitted Name]	Characterization of yap7 in Saccharomyces cerevisiae and the human pathogen Candida glabrata
Experiment Description	This study aimed at characterizing the targets of the bZIP transcription factor Yap7 in two yeast species: Saccharomyces cerevisiae and the human pathogen Candida glabrata. Transcriptome analyses were thus conducted in wild type and yap7 knock out strains using Agilent microarrays.																				
Experimental Design	genetic modification design	reference design	co-expression_design																		
Comment[AEExperimentType]	transcription profiling by array																				
Experimental Factor Name	genotype	strain																			
Experimental Factor Type	genotype	strain																			
Quality Control Type	biological replicate																				
Quality Control Term Source REF	EFO																				
Public Release Date	2014-10-01																				
Person Last Name	Devaux																				
Person First Name	Frederic																				
Person Email	frederic.devaux@upmc.fr																				
Person Phone	33 1 44 27 81 40																				
Person Address	Biologie Computationnelle et Quantitative, UMR 7238 CNRS-UniversitŽ Pierre et Marie Curie, Site des Cordeliers, 15, Rue de l'Ecole de MŽdecine, 75006 Paris, France																				
Person Affiliation	CNRS-UniversitŽ Pierre et Marie Curi																				
Person Roles	submitter																				
Publication Author List	Jawad Merhej, Thierry Delaveau, Juliette Guitard, Benoit Palancade, Christophe Hennequin, Mathilde Garcia, Gaėlle Lelandais, Frederic Devaux																				
Publication Title	Yap7 is a Transcriptional Repressor of Nitric Oxydase in C. glabrata, which arose from Neofunctionalization after Whole Genome Duplication.																				
Publication Status	submitted																				
Protocol Name	P-MTAB-39738	P-MTAB-39739	P-MTAB-39740	P-MTAB-39741	P-MTAB-39742
Protocol Type	growth protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning protocol																
Protocol Description	Cells were grown at 30 degrees in YPD rich media. Cells were then collected by centrifugation. The OD of all cell cultures was around 1.	Total RNAs were extracted from yeast cultures using RNAeasy kit (Qiagen) following the supplier recommendations. 	For microarray analysis, 1 µg of total RNA was reverse transcribed and labeled with Cy5 or Cy3 using the yeast amino-allyl protocol described at http://www.transcriptome.ens.fr/sgdb/protocols/.	labelled cDNAs from the wild type and hpr1 K-R mutant were competitively hybridized on 8x60k yeast Agilent slides using the standard Agilent hybrization protocol.	The array was read using an Agilent G2505C DNA microarray scanner and the TIFF images extracted with the Agilent Feature Extraction software (version 10.5.1.1) using the 20bit coding ability.																
Protocol Term Source REF	EFO	EFO	EFO	EFO	EFO																
Term Source Name	EFO	ArrayExpress
Term Source File	http://www.ebi.ac.uk/efo	http://www.ebi.ac.uk/arrayexpress																			
SDRF File	E-MTAB-2653.sdrf.txt